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Biacore biacore 3000 instrument
Biacore 3000 Instrument, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3000+instrument/3000+biacore+instrument/us12649772-699-9-9
Average 86 stars, based on 1 article reviews
biacore 3000 instrument - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Binding Assay:

Article Title: Antibody variants and uses thereof
Article Snippet: .. As used herein, the term “binding” in the context of the binding of an antibody to a predetermined antigen typically is a binding with an affinity corresponding to a KD of about 10−6 M or less, e.g. 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less, about 10−10 M or less, or about 10−11M or even less when determined by for instance surface plasmon resonance (SPR) technology in a BIAcore 3000 instrument using the antigen as the ligand and the antibody as the analyte, and binds to the predetermined antigen with an affinity corresponding to a KD that is at least ten-fold lower, such as at least 100 fold lower, for instance at least 1,000 fold lower, such as at least 10,000 fold lower, for instance at least 100,000 fold lower than its affinity for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely-related antigen. ..

Article Title: Targeting the Hippo pathway in Schwann cells ameliorates peripheral nerve degeneration via a polypharmacological mechanism
Article Snippet: .. The binding analysis of recombinant QKI to let-7b-biotin was performed as described [ ] by using a Biacore 3000 instrument and BIA evaluation software. .. The 50–100 response units (RU) of let-7b-biotin were immobilized onto a streptavidin chip (SA chip, GE Healthcare) and diluted in a running buffer consisting of 10 mM HEPES, 150 mM Sodium Chloride, 1 mM DTT, and 0.025 % surfactant P20 at pH 7.4.

Article Title: Compositions and methods for producing a composition
Article Snippet: .. Binding of CTLA4-Ig dimer and tetramer to B7-1Ig using surface plasmon resonance: Concentration Analyses: Concentration analyses of the various CTLA4-Ig species to B7-1Ig were performed on a Biacore 3000 instrument (Biacore, Piscataway, NJ) according to Method 7441-42 with minor modifications. .. Modifications include the following: Biacore 3000 was used instead of Biacore C. The flow rate was 10 μL/min instead of 20 μL/min.

Article Title: Antibodies, and bispecific antigen-binding molecules that bind HER2 and/or APLP2, conjugates, and uses thereof
Article Snippet: .. For instance, binding affinity typically corresponds to a KD value of about 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less when determined by, for instance, surface plasmon resonance (SPR) technology in a BIAcore 3000 instrument using the antigen as the ligand and the antibody, Ig, antibody-binding fragment, or Fc-containing protein as the analyte (or antiligand). .. Cell-based binding strategies, such as fluorescent-activated cell sorting (FACS) binding assays, are also routinely used and provide binding characterization data with respect to cell-surface expressed proteins.

SPR Assay:

Article Title: Antibody variants and uses thereof
Article Snippet: .. As used herein, the term “binding” in the context of the binding of an antibody to a predetermined antigen typically is a binding with an affinity corresponding to a KD of about 10−6 M or less, e.g. 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less, about 10−10 M or less, or about 10−11M or even less when determined by for instance surface plasmon resonance (SPR) technology in a BIAcore 3000 instrument using the antigen as the ligand and the antibody as the analyte, and binds to the predetermined antigen with an affinity corresponding to a KD that is at least ten-fold lower, such as at least 100 fold lower, for instance at least 1,000 fold lower, such as at least 10,000 fold lower, for instance at least 100,000 fold lower than its affinity for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely-related antigen. ..

Article Title: Anti-HER2 biparatopic antibody KJ015 has near-native structure, functional balanced high affinity, and synergistic efficacy with anti-PD-1 treatment in vivo
Article Snippet: .. SPR with a BIAcore® 3000 instrument (BIAcore®,Uppsala, Sweden) was utilized to assess the affinity of direct interaction between KJ015, trastuzumab, pertuzumab, and domain II or IV of HER2. .. Antibodies and antigens were immobilized onto a BIAcore CM5 sensor chip via standard amine coupling.

Article Title: Compositions and methods for producing a composition
Article Snippet: .. Binding of CTLA4-Ig dimer and tetramer to B7-1Ig using surface plasmon resonance: Concentration Analyses: Concentration analyses of the various CTLA4-Ig species to B7-1Ig were performed on a Biacore 3000 instrument (Biacore, Piscataway, NJ) according to Method 7441-42 with minor modifications. .. Modifications include the following: Biacore 3000 was used instead of Biacore C. The flow rate was 10 μL/min instead of 20 μL/min.

Article Title: Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system
Article Snippet: .. Surface plasmon resonance was performed on a Biacore 3000 instrument at 25°C. .. Biotinylated dsDNA (SH167 with SH181) was coupled to streptavidin on the sensor surface (GE Healthcare) at equivalent levels, resulting in a 100 to 150 RU increase.

Article Title: Antibodies, and bispecific antigen-binding molecules that bind HER2 and/or APLP2, conjugates, and uses thereof
Article Snippet: .. For instance, binding affinity typically corresponds to a KD value of about 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less when determined by, for instance, surface plasmon resonance (SPR) technology in a BIAcore 3000 instrument using the antigen as the ligand and the antibody, Ig, antibody-binding fragment, or Fc-containing protein as the analyte (or antiligand). .. Cell-based binding strategies, such as fluorescent-activated cell sorting (FACS) binding assays, are also routinely used and provide binding characterization data with respect to cell-surface expressed proteins.

Recombinant:

Article Title: Targeting the Hippo pathway in Schwann cells ameliorates peripheral nerve degeneration via a polypharmacological mechanism
Article Snippet: .. The binding analysis of recombinant QKI to let-7b-biotin was performed as described [ ] by using a Biacore 3000 instrument and BIA evaluation software. .. The 50–100 response units (RU) of let-7b-biotin were immobilized onto a streptavidin chip (SA chip, GE Healthcare) and diluted in a running buffer consisting of 10 mM HEPES, 150 mM Sodium Chloride, 1 mM DTT, and 0.025 % surfactant P20 at pH 7.4.

Software:

Article Title: Targeting the Hippo pathway in Schwann cells ameliorates peripheral nerve degeneration via a polypharmacological mechanism
Article Snippet: .. The binding analysis of recombinant QKI to let-7b-biotin was performed as described [ ] by using a Biacore 3000 instrument and BIA evaluation software. .. The 50–100 response units (RU) of let-7b-biotin were immobilized onto a streptavidin chip (SA chip, GE Healthcare) and diluted in a running buffer consisting of 10 mM HEPES, 150 mM Sodium Chloride, 1 mM DTT, and 0.025 % surfactant P20 at pH 7.4.

Concentration Assay:

Article Title: Compositions and methods for producing a composition
Article Snippet: .. Binding of CTLA4-Ig dimer and tetramer to B7-1Ig using surface plasmon resonance: Concentration Analyses: Concentration analyses of the various CTLA4-Ig species to B7-1Ig were performed on a Biacore 3000 instrument (Biacore, Piscataway, NJ) according to Method 7441-42 with minor modifications. .. Modifications include the following: Biacore 3000 was used instead of Biacore C. The flow rate was 10 μL/min instead of 20 μL/min.



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